CD95 RealYellow 655 (DX2, Mouse) - Reactivity: Baboon, Cynomolgus macaque, Human, Rhesus macaque
| Description |
The DX2 monoclonal antibody specifically binds to the human Fas antigen (also called APO-1). This 45 kDa type I transmembrane glycoprotein was designated as CD95 at the Fifth HLDA Workshop. Fas is a member of the TNF-receptor superfamily and is also known as Tumor necrosis factor receptor superfamily member 6 (TNFRSF6). It is differentially expressed on a variety of normal and neoplastic cells. These include some undifferentiated thymocytes, and activated T and B lymphocytes, natural killer (NK) cells, monocytes, neutrophils, fibroblasts, and cell lines. CD95 is preferentially expressed on CD45RO-positive memory T lymphocytes and γ/δ T lymphocytes. The Fas/CD95 antigen is a polypeptide that plays a role in the programmed sequence of events leading to cell death, termed apoptosis. Crosslinking CD95 with DX2 antibody delivers an apoptotic signal indicating that DX2 recognizes a functional epitope of the CD95 antigen. |
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| Conjugate | RealYellow 655 | |
| Clone | DX2 | |
| Target Species | Baboon, Cynomolgus macaque, Human, Rhesus macaque | |
| Applications | FC | |
| Supplier | BD Biosciences | |
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| Isotype |
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011]
BD Horizon RealYellow 655 (RY655) has an excitation peak at 557 nm and an emission peak at 654 nm. RY655 reagents offer a bright fluorochrome to support the detection of low expression surface and intracellular markers and increase panel design flexibility, offering minimal cross-laser excitation from the 488-nm blue laser in hundreds of antibody specificities.. RY655 is excited primarily by the 561 nm yellow-green laser.
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