FLUOFORTE
FLUOFORTE (Ex-Max 495 nm/Em-Max 515 nm)
FLUOFORTE from Enzo Life Sciences is a cell-permeant reagent used in calcium detection assays similar to Fluo-3, AM and Fluo-4 AM. It has an excitation peak at 495 nm and an emission peak at 515 nm. This reagent can be used in microplate assays, fluorescence microscopy and flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 495 nm
- Emission maximum
- 515 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
FLUOFORTE has an excitation maximum at 495 nm and an emission maximum at 515 nm, a Stokes shift of 20 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is FLUOFORTE?
- FLUOFORTE from Enzo Life Sciences is a cell-permeant reagent used in calcium detection assays similar to Fluo-3, AM and Fluo-4 AM. It has an excitation peak at 495 nm and an emission peak at 515 nm. This reagent can be used in microplate assays, fluorescence microscopy and flow cytometry.
- What laser excites FLUOFORTE?
- FLUOFORTE is optimally excited by the 488 laser line. Its excitation maximum is 495 nm.
- What filter should I use to detect FLUOFORTE?
- A 515/30 filter captures the peak of the FLUOFORTE emission near 515 nm with minimal spillover.