Hoechst (33342)
Hoechst (33342) (Ex-Max 361 nm/Em-Max 497 nm)
Hoechst (33342) is a cell-permeant, fluorogenic nucleic acid stain. It has an excitation peak at 361 nm and an emission peak at 497 nm. It is useful in counter-staining nuclei in both flow cytometry and microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 361 nm
- Emission maximum
- 497 nm
- Optimal laser line
- 355
- Optimal filter
- 450/50
- Commercial antibodies
- 7 from 4 suppliers
Hoechst (33342) has an excitation maximum at 361 nm and an emission maximum at 497 nm, a Stokes shift of 136 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is Hoechst (33342)?
- Hoechst (33342) is a cell-permeant, fluorogenic nucleic acid stain. It has an excitation peak at 361 nm and an emission peak at 497 nm. It is useful in counter-staining nuclei in both flow cytometry and microscopy.
- What laser excites Hoechst (33342)?
- Hoechst (33342) is optimally excited by the 355 laser line. Its excitation maximum is 361 nm.
- What filter should I use to detect Hoechst (33342)?
- A 450/50 filter captures the peak of the Hoechst (33342) emission near 497 nm with minimal spillover.
- How many antibodies are available conjugated to Hoechst (33342)?
- FluoroFinder tracks 7 commercially available Hoechst (33342) conjugates, searchable by target, clone, host and application.