Alexa Fluor 647 Dye Profile


640

Laser Line

660/20

Common Filter

650

Excitation Max

668

Emission Max


Spectra Viewer

About Alexa Fluor 647


Alexa Fluor® 647 (AF647, Alexa 647) is a red-emitting synthetic fluorophore that can be excited by the 640 nm red laser and captured with a 660/20 nm bandpass filter. AF647 has an excitation peak at 650 nm and an emission peak at 668 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor™ 647 (ATT Bioquest), APC and DyLight™ 650 (ThermoFisher Scientific). AF647 is commonly used for flow cytometry, microscopy, super-resolution microscopy as well as imaging applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye. The Alexa Fluor dye family was originally developed by Molecular Probes, now a part of ThermoFisher Scientific. AlexaFluor is a registered trademark of Invitrogen, ThermoFisher Scientific. Alexa Fluor dyes come in a wide variety of modified formats that allow for easy conjugations or right out of the box use.


Popular Alexa Fluor 647 Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
R&D Systems
FAB25381R-100UG
FC DDR2
Human
Mouse
IgG2b
290804
100 μg
$295
View
Sony
3667040
FC,IF H2A.X-Phospho (Ser139)
Human,Mouse
Mouse
IgG1 κ
2F3
100 Tests
$365
View
Novus Biologicals
NB600-344AF647
WB,ICC, IF Flag Tag
Monkey,All Species,Human,Primate
Goat
IgG
0.1 ml
$429
View
Novus Biologicals
NBP1-04676AF647
IHC,Knockout Validated,ICS, FC,WB,FC,IHC-P,ICC, IF,IHC-Fr PGC-1 alpha
Human,Rat,Mouse,Squirrel,Hamster,Porcine,Goat
Rabbit
IgG
0.1 ml
$479
View
R&D Systems
FAB3478R-100UG
FC CCR10
Human
Rat
IgG2a
314305
100 μg
$375
View
Sony
2201065
FC FoxP3
Human,Cynomolgus,Rhesus,Baboon,Chimpanzee
Mouse
IgG1 κ
259D
25 Tests
$162
View
R&D Systems
FAB1125R-100UG
FC TER-119
Mouse
Rat
IgG2b
TER-119
100 μg
$359
View
Sony
1144560
FC CD49b pan-NK cells
Mouse
Rat
IgM κ
DX5
100 μg
$226
View
R&D Systems
FAB17291R-025
FC TREM-2
Human,Mouse
Rat
IgG2b
237920
25 Tests
$189
View
R&D Systems
FAB9927R-100UG
FC CD25
Human
Human
IgG1
Hu102
100 μg
$355
View
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Popular Alexa Fluor 647 Secondary Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
BioLegend
405707
FC,3D-IHC IgD
Mouse
Rat
IgG2a κ
11-26c.2a
25 μg
$105
View
BioLegend
405416
FC,IHC-Fr IgG
Rat
Goat
IgG
Poly4054
100 μg
$176
View
BioLegend
410714
FC IgG
Human
Rat
IgG2a κ
M1310G05
100 Tests
$325
View
BioLegend
407116
FC IgG2a
Mouse
Rat
IgG
RMG2a-62
100 μg
$182
View
BioLegend
348228
FC IgD
Human
Mouse
IgG2a κ
IA6-2
100 Tests
$336
View
BioLegend
405708
FC,3D-IHC IgD
Mouse
Rat
IgG2a κ
11-26c.2a
100 μg
$248
View
BioLegend
406525
FC IgM
Mouse
Rat
IgG2a κ
RMM-1
25 μg
$83
View
BioLegend
405322
FC,IHC-P IgG
Mouse
Goat
IgG
Poly4053
100 μg
$171
View
Thermo Fisher Scientific
A21238
FC,IF IgM
Mouse
Goat
IgG
Polyclonal
250 μl
$139
View
Thermo Fisher Scientific
A21246
FC,IF IgG
Rabbit
Goat
IgG
Polyclonal
250 μl
$139
View
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Alexa Fluor 647 Conjugation Kits


Product Type Company/SKU Applications Size / Price Details
Conjugation Kit Thermo Fisher Scientific
A20186
Labeling 5 x 100 μg
$484
View

Alexa Fluor 647 Conjugation Services


Product Type Company/SKU Applications Details
Conjugation Service Biotium
BIOTIUM-CONJ-AF647
N/A View
Conjugation Service Columbia Biosciences
COLUMBIA-CONJ-Alexa Fluor 647
Labeling View
Conjugation Service BD Biosciences
BD-CONJ-AF647
N/A View
Conjugation Service BioLegend
BL-CONJ-AF647
N/A View