Resazurin
Resazurin (Ex-Max 568 nm/Em-Max 582 nm)
Resazurin is a fluorescent dye used in a variety of assays, namely for cell viability.
Excitation & emission spectra
Key facts
- Excitation maximum
- 568 nm
- Emission maximum
- 582 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
Resazurin has an excitation maximum at 568 nm and an emission maximum at 582 nm, a Stokes shift of 14 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is Resazurin?
- Resazurin is a fluorescent dye used in a variety of assays, namely for cell viability.
- What laser excites Resazurin?
- Resazurin is optimally excited by the 561 laser line. Its excitation maximum is 568 nm.
- What filter should I use to detect Resazurin?
- A 585/42 filter captures the peak of the Resazurin emission near 582 nm with minimal spillover.