IFP 1.4
IFP 1.4 (Ex-Max 679 nm/Em-Max 704 nm)
IFP 1.4 is a monomeric fluorescent protein derived from the bacterium, Deinococcus radiodurans. It has a excitation peak at both 355 nm and 684 nm with an emission peak at 704 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 679 nm
- Emission maximum
- 704 nm
- Optimal laser line
- 355/680
- Optimal filter
- 695/40
- Extinction coefficient
- 92000
- Quantum yield
- 0.07
- Molecular weight
- 34800
IFP 1.4 has an excitation maximum at 679 nm and an emission maximum at 704 nm, a Stokes shift of 25 nm. Its extinction coefficient is 92000 M⁻¹cm⁻¹. It is optimally excited by the 355/680 laser line.
Frequently asked questions
- What is IFP 1.4?
- IFP 1.4 is a monomeric fluorescent protein derived from the bacterium, Deinococcus radiodurans. It has a excitation peak at both 355 nm and 684 nm with an emission peak at 704 nm.
- What laser excites IFP 1.4?
- IFP 1.4 is optimally excited by the 355/680 laser line. Its excitation maximum is 679 nm.
- What filter should I use to detect IFP 1.4?
- A 695/40 filter captures the peak of the IFP 1.4 emission near 704 nm with minimal spillover.