CellTrace Violet
CellTrace Violet (Ex-Max 405 nm/Em-Max 450 nm)
CellTrace® Violet Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. This reagent passively crosses the cell membrane and is hydrolyzed causing it to become fluorescent and retained intracellularly. It then covalently crosslinks with primary amine-containing proteins inside the cell. With each daughter generation, as the cell divides, the intensity of the dye will become half of it's parent, indicating a symmetrical division of the cell and cytoplasm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 405 nm
- Emission maximum
- 450 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Commercial antibodies
- 2 from 1 supplier
CellTrace Violet has an excitation maximum at 405 nm and an emission maximum at 450 nm, a Stokes shift of 45 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is CellTrace Violet?
- CellTrace® Violet Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry. This reagent passively crosses the cell membrane and is hydrolyzed causing it to become fluorescent and retained intracellularly. It then covalently crosslinks with primary amine-containing proteins inside the cell. With each daughter generation, as the cell divides, the intensity of the dye will become half of it's parent, indicating a symmetrical division of the cell and cytoplasm.
- What laser excites CellTrace Violet?
- CellTrace Violet is optimally excited by the 405 laser line. Its excitation maximum is 405 nm.
- What filter should I use to detect CellTrace Violet?
- A 450/50 filter captures the peak of the CellTrace Violet emission near 450 nm with minimal spillover.
- How many antibodies are available conjugated to CellTrace Violet?
- FluoroFinder tracks 2 commercially available CellTrace Violet conjugates, searchable by target, clone, host and application.