DyLight 633
DyLight™ 633 (Ex-Max 638 nm/Em-Max 658 nm)
DyLight™ 633 is a red-emitting fluroescent dye that can be excited by the 628 nm laser and collected using a 670/30 nm bandpass filter. DyLight™ 633 has an excitation peak of 638 nm and an emission peak of 658 nm, and is spectrally similar to Alexa Fluor™ 633. DyLight™ 633 is most commonly used in flow cytometery and fluorescence microscopy applications.
Excitation & emission spectra
Key facts
- Excitation maximum
- 638 nm
- Emission maximum
- 658 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Relative brightness
- 3 of 5
- Extinction coefficient
- 170000
- Commercial conjugates
- 8 from 6 suppliers
DyLight 633 has an excitation maximum at 638 nm and an emission maximum at 658 nm, a Stokes shift of 20 nm. Its extinction coefficient is 170000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is DyLight 633?
- DyLight™ 633 is a red-emitting fluroescent dye that can be excited by the 628 nm laser and collected using a 670/30 nm bandpass filter. DyLight™ 633 has an excitation peak of 638 nm and an emission peak of 658 nm, and is spectrally similar to Alexa Fluor™ 633. DyLight™ 633 is most commonly used in flow cytometery and fluorescence microscopy applications.
- What laser excites DyLight 633?
- DyLight 633 is optimally excited by the 628-640 laser line. Its excitation maximum is 638 nm.
- What filter should I use to detect DyLight 633?
- A 660/20 filter captures the peak of the DyLight 633 emission near 658 nm with minimal spillover.
- How bright is DyLight 633?
- DyLight 633 has a relative brightness of 3 out of 5.
- How many antibodies are available conjugated to DyLight 633?
- FluoroFinder tracks 8 commercially available DyLight 633 conjugates, searchable by target, clone, host and application.