PE-eFluor 610
PE-eFluor™ 610 (Ex-Max 561 nm/Em-Max 607 nm)
PE-eFluor™ 610 (PE-eF610, RPE-eF610) from Thermo Fisher Scientific is an orange-emitting tandem fluorophore that combines pycoerythrin (PE) and eFluor™ 610. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, eF610, which emitts light that can be captured with a 610/20 nm bandpass filter. PE-eF610 has an excitation peak at 565 nm and an emission peak at 607 nm, and is suitable alternative to PE-Texas Red®. PE-eF610 is mainly used for flow cytometry. This tandem is often a great option because of PE is excitable by multiple lasers due to its broad excitation spectrum and the eFluor™ 610 acceptor molecule emits brightly in the Orange/Red spectrum. Many cytometers are commonly set up with the 610/20 bandpass filter that can detect this fluorophore's emission. This dye is part of the larger eFluor™ dye family, developed by eBioscience which is now part of ThermoFisher Scientific.
Excitation & emission spectra
Key facts
- Excitation maximum
- 565 nm
- Emission maximum
- 607 nm
- Optimal laser line
- 488-561
- Optimal filter
- 610/20
- Relative brightness
- 4 of 5
- Commercial antibodies
- 230 from 2 suppliers
PE-eFluor 610 has an excitation maximum at 565 nm and an emission maximum at 607 nm, a Stokes shift of 42 nm. It is optimally excited by the 488-561 laser line.
Frequently asked questions
- What is PE-eFluor 610?
- PE-eFluor™ 610 (PE-eF610, RPE-eF610) from Thermo Fisher Scientific is an orange-emitting tandem fluorophore that combines pycoerythrin (PE) and eFluor™ 610. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, eF610, which emitts light that can be captured with a 610/20 nm bandpass filter. PE-eF610 has an excitation peak at 565 nm and an emission peak at 607 nm, and is suitable alternative to PE-Texas Red®. PE-eF610 is mainly used for flow cytometry. This tandem is often a great option because of PE is excitable by multiple lasers due to its broad excitation spectrum and the eFluor™ 610 acceptor molecule emits brightly in the Orange/Red spectrum. Many cytometers are commonly set up with the 610/20 bandpass filter that can detect this fluorophore's emission. This dye is part of the larger eFluor™ dye family, developed by eBioscience which is now part of ThermoFisher Scientific.
- What laser excites PE-eFluor 610?
- PE-eFluor 610 is optimally excited by the 488-561 laser line. Its excitation maximum is 565 nm.
- What filter should I use to detect PE-eFluor 610?
- A 610/20 filter captures the peak of the PE-eFluor 610 emission near 607 nm with minimal spillover.
- How bright is PE-eFluor 610?
- PE-eFluor 610 has a relative brightness of 4 out of 5.
- How many antibodies are available conjugated to PE-eFluor 610?
- FluoroFinder tracks 230 commercially available PE-eFluor 610 conjugates, searchable by target, clone, host and application.