About Propidium Iodide

---

Propidium Iodide (PI) is an orange/red-emitting DNA intercalating dye that can be excited by the 532 nm green laser and captured with a 630/69 nm bandpass filter. Unbound, PI has an excitation peak at 493 nm and an emission peak at 636 nm. However, when PI is bound to DNA the excitiaton peak shifts to 535 nm and the emission peak shifts to 617 nm. PI is predominantly used in flow cytometry as a DNA stain evaluate cell viability or to monitor cell cycle analysis, or in microscopy to visualize the nucleus and other DNA-containing organelles. PI is a generic dye that is sold by multiple suppliers.

Dyes similar to Propidium Iodide based on emission

Check to add to spectra viewer

Alexa Fluor 594 Alexa Fluor 610 ATTO RHO101 ATTO Rho13 BDY TR-X SE BODIPY TR Brilliant Ultraviolet 615 CF583R CF594 CF594ST CF597R CoraLite 594 Cardinal Red CyTRAK Orange DY-350XL DyLight 594 ECD eFluor 615 eFluor 625NC Ethidium B. Ethidium Homodimer I Ethidium Homodimer III EvaRuby Flash Phalloidin Red 594 FVS620 Hc Red HiLyte 594 Janelia Fluor 585 LIVE 460L LIVE 590 Live/Dead Fix Red Live-or-Dye 594/614 LSS-mKate1 LysoBrite Deep Red MagDot 610 Magic Red mCherry MemBrite Fix 594/615 mKeima mRaspberry MultiDot 610 NovaFluor Blue 610 / 30S NovaFluor Blue 610 / 70S NovaFluor Yellow 610 PE-CF594 PE-Dazzle 594 PE-DyLight 594 PE-Texas Red PE-Vio615 PhenoVue Fluor 594 PMA PMAxx PromoFluor 594 Protoporphyrin IX Qdot 625 ReadiLink 594-610 STAR 460L STAR 470SXP STAR 520SXP StarBright Blue 615 STAR ORANGE SuperNova v605 SureLight PE-594 Texas Red TFAX 594 SE Zombie Red XFD594 RealYellow 610 ABflo 610 RealBlue 613 ATTO 590