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NucSpot 500/515

NucSpot 500/515 has an excitation peak at 497 nm and an emission peak at 513 nm. NucSpot Nuclear Stains are cell membrane-impermeant, nuclear-specific counterstains. They have minimal fluorescence until they bind to DNA and can be used for no-wash nuclear staining. They can also be used for selective staining of dead cells in unfixed cell cultures for analysis by flow cytometry or fluorescence imaging.

Excitation & emission spectra

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Key facts

Excitation maximum
497 nm
Emission maximum
513 nm

NucSpot 500/515 has an excitation maximum at 497 nm and an emission maximum at 513 nm, a Stokes shift of 16 nm.

Frequently asked questions

What is NucSpot 500/515?
NucSpot 500/515 has an excitation peak at 497 nm and an emission peak at 513 nm. NucSpot Nuclear Stains are cell membrane-impermeant, nuclear-specific counterstains. They have minimal fluorescence until they bind to DNA and can be used for no-wash nuclear staining. They can also be used for selective staining of dead cells in unfixed cell cultures for analysis by flow cytometry or fluorescence imaging.