Cyan 500 NHS-Ester
Cyan 500 NHS-Ester is an amine-reactive fluorescent label. It is designed and recommended by Roche for application in LightCycler® instruments. The dye is used to label amino-modified oligonucleotides which can be detected on excitation at 430 nm in Roche LightCycler® instruments. Cyan 500 NHS-Ester can also be used as label for other biomolecules such as proteins etc. The dye has a molecular weight of 580 g/mol and is moderately hydrophilic.
Excitation & emission spectra
Key facts
- Excitation maximum
- 450 nm
- Emission maximum
- 495 nm
- Optimal laser line
- 445
- Relative brightness
- 1 of 5
- Extinction coefficient
- 45000
- Quantum yield
- .85 (PBS, pH 7.4)
- Molecular weight
- 580
Cyan 500 NHS-Ester has an excitation maximum at 450 nm and an emission maximum at 495 nm, a Stokes shift of 45 nm. Its extinction coefficient is 45000 M⁻¹cm⁻¹. It is optimally excited by the 445 laser line.
Frequently asked questions
- What is Cyan 500 NHS-Ester?
- Cyan 500 NHS-Ester is an amine-reactive fluorescent label. It is designed and recommended by Roche for application in LightCycler® instruments. The dye is used to label amino-modified oligonucleotides which can be detected on excitation at 430 nm in Roche LightCycler® instruments. Cyan 500 NHS-Ester can also be used as label for other biomolecules such as proteins etc. The dye has a molecular weight of 580 g/mol and is moderately hydrophilic.
- What laser excites Cyan 500 NHS-Ester?
- Cyan 500 NHS-Ester is optimally excited by the 445 laser line. Its excitation maximum is 450 nm.
- How bright is Cyan 500 NHS-Ester?
- Cyan 500 NHS-Ester has a relative brightness of 1 out of 5 (quantum yield .85).