Sytox Deep Red
Sytox Deep Red (Ex-Max 657 nm/Em-Max 678 nm)
Sytox Deep Red from Thermo Fisher Scientific is a cell-impermeant, fluorogenic nucleic acid stain that can be used as a viability dye for use in flow cytometry and as a nuclear counterstain in fluorescence microscopy. It has an excitation peak at 660 nm and an emission peak at 680 nm and is often used in spectral flow cytometry due to these unique spectral characteristics.
Excitation & emission spectra
Key facts
- Excitation maximum
- 657 nm
- Emission maximum
- 678 nm
- Optimal laser line
- 628-640
- Optimal filter
- 695/40
Sytox Deep Red has an excitation maximum at 657 nm and an emission maximum at 678 nm, a Stokes shift of 21 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Sytox Deep Red?
- Sytox Deep Red from Thermo Fisher Scientific is a cell-impermeant, fluorogenic nucleic acid stain that can be used as a viability dye for use in flow cytometry and as a nuclear counterstain in fluorescence microscopy. It has an excitation peak at 660 nm and an emission peak at 680 nm and is often used in spectral flow cytometry due to these unique spectral characteristics.
- What laser excites Sytox Deep Red?
- Sytox Deep Red is optimally excited by the 628-640 laser line. Its excitation maximum is 657 nm.
- What filter should I use to detect Sytox Deep Red?
- A 695/40 filter captures the peak of the Sytox Deep Red emission near 678 nm with minimal spillover.