Calcein Blue
Calcein Blue (Ex-Max 359 nm/Em-Max 445 nm)
Calcein Blue is the hydrodlyzed version of Calcein, Blue AM. It is commonly used as a cell impermeant, esterase independet control for Calcein Blue, AM. Commonly used in fluorescence microscopy, flow cytometry, or fluorescent microplate reader assays. It has an excitation peak at 359 nm and an emission peak at 445 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 359 nm
- Emission maximum
- 445 nm
- Optimal laser line
- 355
- Optimal filter
- 450/50
- Molecular weight
- 994.9
Calcein Blue has an excitation maximum at 359 nm and an emission maximum at 445 nm, a Stokes shift of 86 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is Calcein Blue?
- Calcein Blue is the hydrodlyzed version of Calcein, Blue AM. It is commonly used as a cell impermeant, esterase independet control for Calcein Blue, AM. Commonly used in fluorescence microscopy, flow cytometry, or fluorescent microplate reader assays. It has an excitation peak at 359 nm and an emission peak at 445 nm.
- What laser excites Calcein Blue?
- Calcein Blue is optimally excited by the 355 laser line. Its excitation maximum is 359 nm.
- What filter should I use to detect Calcein Blue?
- A 450/50 filter captures the peak of the Calcein Blue emission near 445 nm with minimal spillover.