Loading the Dye Directory…

BactoView Live Green

BactoView Live Green (Ex-Max 498 nm/Em-Max 522 nm)

BactoView Live Green from Biotium stains both live and dead bacteria, similar to SYTO dyes from Thermo Fisher Scientific. BactoView Live Green has an excitation peak at 489 nm and an emission peak at 522 nm.

Excitation & emission spectra

400450500550600650700750800

Key facts

Excitation maximum
498 nm
Emission maximum
522 nm
Optimal laser line
488
Optimal filter
525/50

BactoView Live Green has an excitation maximum at 498 nm and an emission maximum at 522 nm, a Stokes shift of 24 nm. It is optimally excited by the 488 laser line.

Frequently asked questions

What is BactoView Live Green?
BactoView Live Green from Biotium stains both live and dead bacteria, similar to SYTO dyes from Thermo Fisher Scientific. BactoView Live Green has an excitation peak at 489 nm and an emission peak at 522 nm.
What laser excites BactoView Live Green?
BactoView Live Green is optimally excited by the 488 laser line. Its excitation maximum is 498 nm.
What filter should I use to detect BactoView Live Green?
A 525/50 filter captures the peak of the BactoView Live Green emission near 522 nm with minimal spillover.