LysoView 650
LysoView™ 650 (Ex-Max 655 nm/Em-Max 681 nm)
Biotium's LysoView™ dyes are cell-permeant alkaline probes that localize to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoView™ 650 has an excitation peak at 655 nm and an emission peak at 681 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 655 nm
- Emission maximum
- 681 nm
- Optimal laser line
- 628-640
- Optimal filter
- 695/40
- Commercial antibodies
- 2 from 1 supplier
LysoView 650 has an excitation maximum at 655 nm and an emission maximum at 681 nm, a Stokes shift of 26 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is LysoView 650?
- Biotium's LysoView™ dyes are cell-permeant alkaline probes that localize to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoView™ 650 has an excitation peak at 655 nm and an emission peak at 681 nm.
- What laser excites LysoView 650?
- LysoView 650 is optimally excited by the 628-640 laser line. Its excitation maximum is 655 nm.
- What filter should I use to detect LysoView 650?
- A 695/40 filter captures the peak of the LysoView 650 emission near 681 nm with minimal spillover.
- How many antibodies are available conjugated to LysoView 650?
- FluoroFinder tracks 2 commercially available LysoView 650 conjugates, searchable by target, clone, host and application.