About SNARF 1 pH6.0

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SNARF-1, AM is a cell-permeant pH probe with a pKa of 7.5 after hydrolysis of the acetoxymethyl ester of the probe upon entering live cells. SNARF-1 is a ratiometric indicator sensitive to measuring pH changes between pH 7 and pH 8 and shifts from 580 nm under acidic pH to 640 nm under basic conditions. It also undergoes a shift in its excitation peak between pH6 to pH9 from ~532 nm at pH6 to ~561nm at pH9, but typically this probe is typically used in fluorescent imaging applications with a single excitation peak at 555nm to monitor the dual emission shift.

Dyes similar to SNARF 1 pH6.0 based on emission

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10-Acetyl-37-dihydroxyphenoxazin 5-TAMRA 6-TAMRA alamarBlue Alizarin Red Amplex Red ATTO 550 ATTO 565 ATTO Rho3B Calcein Red-Orange AM CellBrite Steady 550 CellTrace Yellow CF550R CF568 CF568ST CF570 CMA-Chromomycin A3 Cy3.5 CytoTrace Red DsRed2 dsRed DsRed Express dTomato DY-510XL DyLight 549 DyLight 550 eFluor 585NC EMA ExoBrite 560/585 EV Membrane Stain FVS570 GelRed Kaede (red) KikGR1 (red) LipidSpot 488 LIVE 560 Live-or-Dye 568/583 Live-or-Dye NucFix Red MagDot 575 mApple MaxLight 550 MemBrite Fix 568/580 MITO-ID MP (high potential) mScarlet mTangerine MultiDot 575 NeuroVue Red NovaFluor Blue 585 NovaFluor Yellow 590 PE PhenoVue 512 Nucleic Acid Stain PhenoVue 577 Lysosomal Stain PhenoVue Cal-590 AM Bright, Calcium Indicator PhenoVue Cal-590 AM, Calcium Indicator PhenoVue Fluor 400LS PhenoVue Fluor 555 Live Cell pHrodo Red PROTEOSTAT-Red Qdot 585 Resazurin Resorufin RFP Rhod-5N (Ca2+ bound) Rhodamine Rhodamine Red-X RealYellow 586 Seta-400-NHS Spark YG 581 Spark YG 593 StarBright Blue 580 StarBright Yellow 575 SureLight RPE TagRFP tdTomato TRITC TSA Vivid 570 VRDye 549 JFX 554, Maleimide BactoView Dead 570/585 Taxol Janelia Fluor 549 CytoLiner 570/590 RayBright PE cFluor YG584 ExoBrite 555/575 True EV Membrane Stain NucSpot 568/580