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POPO-1

POPO-1 (Ex-Max 434 nm/Em-Max 456 nm)

POPO-1 is a cell-impermeant, fluorogenic bis-intercalating nucleic acid stain. POPO-1 has an excitation peak at 433 nm and an emission peak at 457 nm. It is commonly used as a dead and apoptotic cell stain in the following applications: Fluorescence Microscopy.

Excitation & emission spectra

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Key facts

Excitation maximum
434 nm
Emission maximum
456 nm
Optimal laser line
405
Optimal filter
450/50

POPO-1 has an excitation maximum at 434 nm and an emission maximum at 456 nm, a Stokes shift of 22 nm. It is optimally excited by the 405 laser line.

Frequently asked questions

What is POPO-1?
POPO-1 is a cell-impermeant, fluorogenic bis-intercalating nucleic acid stain. POPO-1 has an excitation peak at 433 nm and an emission peak at 457 nm. It is commonly used as a dead and apoptotic cell stain in the following applications: Fluorescence Microscopy.
What laser excites POPO-1?
POPO-1 is optimally excited by the 405 laser line. Its excitation maximum is 434 nm.
What filter should I use to detect POPO-1?
A 450/50 filter captures the peak of the POPO-1 emission near 456 nm with minimal spillover.