Vio Bright 667
Vio®Bright 667 (Ex-Max 645 nm/Em-Max 668 nm)
Vio®Bright® R667 from Miltenyi has an excitation peak at 635 nm and an emission peak at 668. It is spectrally comparable to Cy5 and Alexa Fluor™ 647 in flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 645 nm
- Emission maximum
- 668 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Relative brightness
- 5 of 5
Vio Bright 667 has an excitation maximum at 645 nm and an emission maximum at 668 nm, a Stokes shift of 23 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Vio Bright 667?
- Vio®Bright® R667 from Miltenyi has an excitation peak at 635 nm and an emission peak at 668. It is spectrally comparable to Cy5 and Alexa Fluor™ 647 in flow cytometry.
- What laser excites Vio Bright 667?
- Vio Bright 667 is optimally excited by the 628-640 laser line. Its excitation maximum is 645 nm.
- What filter should I use to detect Vio Bright 667?
- A 660/20 filter captures the peak of the Vio Bright 667 emission near 668 nm with minimal spillover.
- How bright is Vio Bright 667?
- Vio Bright 667 has a relative brightness of 5 out of 5.