iRFP 682
iRFP 682 (Ex-Max 663 nm/Em-Max 682 nm)
iRFP682 is a dimeric fluorescent protein derived from the proteobacteria, Rhodopseudomonas palustris. It has an excitation peak at 663 nm and an emission peak at 682 nm. It requires the cofactor biliverdin for fluorescence.
Excitation & emission spectra
Key facts
- Excitation maximum
- 663 nm
- Emission maximum
- 682 nm
- Optimal laser line
- 628-640
- Optimal filter
- 670/30
- Extinction coefficient
- 90000
- Quantum yield
- 0.11
- Molecular weight
- 34500
iRFP 682 has an excitation maximum at 663 nm and an emission maximum at 682 nm, a Stokes shift of 19 nm. Its extinction coefficient is 90000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is iRFP 682?
- iRFP682 is a dimeric fluorescent protein derived from the proteobacteria, Rhodopseudomonas palustris. It has an excitation peak at 663 nm and an emission peak at 682 nm. It requires the cofactor biliverdin for fluorescence.
- What laser excites iRFP 682?
- iRFP 682 is optimally excited by the 628-640 laser line. Its excitation maximum is 663 nm.
- What filter should I use to detect iRFP 682?
- A 670/30 filter captures the peak of the iRFP 682 emission near 682 nm with minimal spillover.