Alexa Fluor 546 Dye Profile


532

Laser Line

575/36

Common Filter

556

Excitation Max

573

Emission Max


About Alexa Fluor 546


Alexa Fluor® 546 (AF546, Alexa 546) is a yellow-emitting synthetic fluorophore that can be excited by the 532 nm yellow laser and captured with a 575/36 nm bandpass filter. AF532 has an excitation peak at 556 nm and an emission peak at 573 nm, and is spectrally similar to Cy®3 (GE Healthcare), TRITC and iFluor™ 555 (ATT Bioquest). It is uncommon for flow cytometers to be equipped with 532 lasers, so while AF532 can be used for flow cytometry, it is less popular for this application. This dye is commonly used for immunohistochemistry applications and more specifically super-resolution microscopy because it is bright, photostable, and pH insensitive. The Alexa Fluor dye family was originally developed by Molecular Probes, now a part of ThermoFisher Scientific. AlexaFluor is a registered trademark of Invitrogen, ThermoFisher Scientific. Alexa Fluor dyes come in a wide variety of modified formats that allow for easy conjugations or right out of the box use.



Spectra


Popular Alexa Fluor 546 Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
Thermo Fisher Scientific
A20183
Labeling Alexa Fluor 546
N/A
N/A
N/A
5 x 100 μg
$473
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Popular Alexa Fluor 546 Secondary Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
Novus Biologicals
DDXCH04A546-100
ICS, FC,IF,FC IgG4
Human
Human
IgG4
0.1 mg
$302
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Thermo Fisher Scientific
A11035
IF,FC IgG
Rabbit
Goat
IgG
Polyclonal
500 μl
$199
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Thermo Fisher Scientific
A-21089
IF,FC IgG
Human
Goat
IgG
Polyclonal
500 μl
$165
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Alexa Fluor 546 Conjugation Kits


Product Type Company/SKU Applications Size / Price Details
Conjugation Kit Thermo Fisher Scientific
A20183
Labeling 5 x 100 μg
$473
View

Alexa Fluor 546 Conjugation Services


Product Type Company/SKU Applications Details
Conjugation Service Biotium
BIOTIUM-CONJ-AF546
N/A View