About Alexa Fluor 546

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Alexa Fluor® 546 (AF546, Alexa 546) is a yellow-emitting synthetic fluorophore that can be excited by the 532 nm yellow laser and captured with a 575/36 nm bandpass filter. AF532 has an excitation peak at 556 nm and an emission peak at 573 nm, and is spectrally similar to Cy®3 (GE Healthcare), TRITC and iFluor™ 555 (ATT Bioquest). It is uncommon for flow cytometers to be equipped with 532 lasers, so while AF532 can be used for flow cytometry, it is less popular for this application. This dye is commonly used for immunohistochemistry applications and more specifically super-resolution microscopy because it is bright, photostable, and pH insensitive. The Alexa Fluor dye family was originally developed by Molecular Probes, now a part of ThermoFisher Scientific. AlexaFluor is a registered trademark of Invitrogen, ThermoFisher Scientific. Alexa Fluor dyes come in a wide variety of modified formats that allow for easy conjugations or right out of the box use.

Dyes similar to Alexa Fluor 546 based on emission

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5-TAMRA 6-TAMRA alamarBlue Alexa Fluor 555 Amplex Red ATTO 550 BDY TMR-X SE BODIPY 558/568 BODIPY TMR-X BPE Brilliant Violet 570 Calcein Red AM Calcein Red Calcium Orange CellBrite Fix 555 CellBrite Orange CellBrite Steady 550 CellMask Orange Plasma Membrane Stain CellROX Orange CellTrace Yellow CellTracker Orange CF535ST CF540 CF550R CF555 CMA-Chromomycin A3 CoraLite Plus 555 Cy3 Cyanine 555 CytoTrace Red DiI dTomato DY-395XL DyLight 549 DyLight 550 Dyomics 547 eFluor 565NC eFluor 570 FVS570 FVS575V HiLyte 555 iFluor 555 Janelia Fluor 549 Kaede (red) LSSmOrange LysoView 550 MagDot 575 MaxLight 550 MITO-ID Green MITO-ID MP (high potential) MitoLite Orange FX570 MitoTracker Orange MultiDot 575 NeuroVue Orange Nile Red NL 557 NovaFluor Yellow 570 OC515 OFP Oyster 550 PE PhenoVue 551 Mitochondrial Stain PhenoVue Fluor 555 PKH26 POPO-3 PromoFluor 555P Qdot 565 ReadiLink 555-570 Resazurin Rhod-2 (Ca2+ bound) Rhod-5N (Ca2+ bound) Rhodamine B Rhodamine Seta-400-NHS Seta-555-NHS Spark Blue 574 Spark YG 570 Spark YG 581 StarBright UltraViolet 575 StarBright Violet 570 SureLight BPE SureLight RPE Synapto Green Sytox Orange TagRFP tdTomato TFAX 546 SE TMRE TRITC ViaKrome 561 VivaFix 547/573 VRDye 549 Zombie Yellow