Janelia Fluor 646
Janelia Fluor® 646 (Ex-Max 646 nm/Em-Max 664 nm)
Janelia Fluor® 646 was developed at the Janelia Campus of the Howard Hughes Medical Institute but is commercialized by other vendors. The Janelia Fluor®s family is unique in that the fluorophores are cell-permeable and are available in photoactivatable forms. These fluorophores were developed for super-resolution microscopy (STED, PALM and STORM) and live-cell microscopy in the HaloTag and SNAP-tag versions. Janelia Fluor® 646 has an excitation peak at 646 nm and an emission peak at 664 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 646 nm
- Emission maximum
- 664 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Relative brightness
- 4 of 5
- Extinction coefficient
- 152000
- Quantum yield
- 0.54
- Commercial conjugates
- 2.5k from 1 supplier
Janelia Fluor 646 has an excitation maximum at 646 nm and an emission maximum at 664 nm, a Stokes shift of 18 nm. Its extinction coefficient is 152000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Janelia Fluor 646?
- Janelia Fluor® 646 was developed at the Janelia Campus of the Howard Hughes Medical Institute but is commercialized by other vendors. The Janelia Fluor®s family is unique in that the fluorophores are cell-permeable and are available in photoactivatable forms. These fluorophores were developed for super-resolution microscopy (STED, PALM and STORM) and live-cell microscopy in the HaloTag and SNAP-tag versions. Janelia Fluor® 646 has an excitation peak at 646 nm and an emission peak at 664 nm.
- What laser excites Janelia Fluor 646?
- Janelia Fluor 646 is optimally excited by the 628-640 laser line. Its excitation maximum is 646 nm.
- What filter should I use to detect Janelia Fluor 646?
- A 660/20 filter captures the peak of the Janelia Fluor 646 emission near 664 nm with minimal spillover.
- How bright is Janelia Fluor 646?
- Janelia Fluor 646 has a relative brightness of 4 out of 5 (quantum yield 0.54).
- How many antibodies are available conjugated to Janelia Fluor 646?
- FluoroFinder tracks 2459 commercially available Janelia Fluor 646 conjugates, searchable by target, clone, host and application.