Carboxyfluorescein
Carboxyfluorescein (Ex-Max 495 nm/Em-Max 515 nm)
Carboxyfluorescein also called Fluorescein or FITC is a generic fluor with an excitation peak at 495 nm and an emission peak at 515 nm. Superior alternatives are Alexa Fluor™ 488, CF®488A, Vio®Bright 488 and DyLight™ 488.
Excitation & emission spectra
Key facts
- Excitation maximum
- 495 nm
- Emission maximum
- 515 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Relative brightness
- 2 of 5
- Commercial conjugates
- 1 from 3 suppliers
Carboxyfluorescein has an excitation maximum at 495 nm and an emission maximum at 515 nm, a Stokes shift of 20 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Carboxyfluorescein?
- Carboxyfluorescein also called Fluorescein or FITC is a generic fluor with an excitation peak at 495 nm and an emission peak at 515 nm. Superior alternatives are Alexa Fluor™ 488, CF®488A, Vio®Bright 488 and DyLight™ 488.
- What laser excites Carboxyfluorescein?
- Carboxyfluorescein is optimally excited by the 488 laser line. Its excitation maximum is 495 nm.
- What filter should I use to detect Carboxyfluorescein?
- A 515/30 filter captures the peak of the Carboxyfluorescein emission near 515 nm with minimal spillover.
- How bright is Carboxyfluorescein?
- Carboxyfluorescein has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to Carboxyfluorescein?
- FluoroFinder tracks 1 commercially available Carboxyfluorescein conjugates, searchable by target, clone, host and application.