CF430
CF®430 (Ex-Max 426 nm/Em-Max 498 nm)
CF®430 from Biotium is a fluorophore with an excitation peak at 426 nm and an emission peak at 498 nm. It is spectrally similar to CF®440 and Alexa Fluor™ 430. Due to its unique spectral characteristics, it can be used in spectral flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 426 nm
- Emission maximum
- 498 nm
- Optimal laser line
- 405
- Optimal filter
- 515/30
- Relative brightness
- 1 of 5
- Extinction coefficient
- 40000
- Molecular weight
- 429
- Commercial conjugates
- 4 from 1 supplier
CF430 has an excitation maximum at 426 nm and an emission maximum at 498 nm, a Stokes shift of 72 nm. Its extinction coefficient is 40000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is CF430?
- CF®430 from Biotium is a fluorophore with an excitation peak at 426 nm and an emission peak at 498 nm. It is spectrally similar to CF®440 and Alexa Fluor™ 430. Due to its unique spectral characteristics, it can be used in spectral flow cytometry.
- What laser excites CF430?
- CF430 is optimally excited by the 405 laser line. Its excitation maximum is 426 nm.
- What filter should I use to detect CF430?
- A 515/30 filter captures the peak of the CF430 emission near 498 nm with minimal spillover.
- How bright is CF430?
- CF430 has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to CF430?
- FluoroFinder tracks 4 commercially available CF430 conjugates, searchable by target, clone, host and application.