VivaFix 649/660
VivaFix 649/660 (Ex-Max 649 nm/Em-Max 660 nm)
VivaFix 649/660 from Bio-Rad is a fixable viability dye useful in flow cytometry in applications where the cells will be fixed and permeabilized. It has an excitation peak at 649 nm and an emission peak at 660 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 649 nm
- Emission maximum
- 660 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
VivaFix 649/660 has an excitation maximum at 649 nm and an emission maximum at 660 nm, a Stokes shift of 11 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is VivaFix 649/660?
- VivaFix 649/660 from Bio-Rad is a fixable viability dye useful in flow cytometry in applications where the cells will be fixed and permeabilized. It has an excitation peak at 649 nm and an emission peak at 660 nm.
- What laser excites VivaFix 649/660?
- VivaFix 649/660 is optimally excited by the 628-640 laser line. Its excitation maximum is 649 nm.
- What filter should I use to detect VivaFix 649/660?
- A 660/20 filter captures the peak of the VivaFix 649/660 emission near 660 nm with minimal spillover.