About Brilliant Violet 570

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Brilliant Violet™ 570 (BV570) is a yellow-emitting tandem fluorophore that can be excited by the 405 nm violet laser and collected using a 585/42 bandpass filter. BV570 has an excitation peak at 405 nm and an emission peak at 570 nm, and is spectrally similar to Pacific Orange and Cascade Yellow (ThermoFisher Scientific). BV570 is not very bright and is most often used in flow cytometry, specifically, it is an alternative to nanocrystals used for intracellular flow. This dye is part of the Brilliant Violet dye line. The Brilliant Violet dye line itself is very popular because other dyes in the line also use the Brilliant Violet 421 core as the donor molecule, while varied acceptor molecules emit light at various points across the visible light spectrum. This allows for multiple dyes to be compatible while excited by the single laser. In cytometry, a user may populate all the available channels off their 405-violet laser with Brilliant Violet dyes.

Dyes similar to Brilliant Violet 570 based on emission

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5-TAMRA 6-TAMRA Alexa Fluor 546 ATTO 542 ATTO 550 BDY TMR-X SE BODIPY 558/568 BODIPY TMR-X BPE Brilliant Ultraviolet 563 Calcein Red AM Calcein Red Calcium Orange CellBrite Fix 555 CellBrite Orange CellBrite Steady 550 CellMask Orange Plasma Membrane Stain CellROX Orange CellTracker Orange CF535ST CF540 CF550R CF555 Chromeo 546 CMA-Chromomycin A3 CoraLite Plus 555 Cy3 Cyanine 555 CytoTrace Red DiI DY-395XL DyLight 549 DyLight 550 Dyomics 547 eFluor 565NC eFluor 570 FVS570 FVS575V HiLyte 555 iFluor 555 Janelia Fluor 549 LSSmOrange LysoView 550 MaxLight 550 MemBrite Fix 543/560 MITO-ID Green MITO-ID MP (high potential) MitoLite Orange FX570 MitoTracker Orange mKusabira-Orange2 NeuroVue Orange Nile Red NL 557 NovaFluor Yellow 570 NucView 530 OC515 OFP Oyster 550 PE PhenoVue 551 Mitochondrial Stain PhenoVue Fluor 555 PKH26 POPO-3 PromoFluor 555P Pyronin Y Qdot 565 ReadiLink 555-570 Rhod-2 (Ca2+ bound) Rhod-5N (Ca2+ bound) Rhodamine B Rhodamine Seta-400-NHS Seta-555-NHS Spark Blue 574 Spark YG 570 StarBright UltraViolet 575 StarBright Violet 570 SureLight BPE SureLight RPE Synapto Green Sytox Orange TFAX 546 SE TMRE TRITC ViaKrome 561 VivaFix 547/573 VRDye 549 Zombie Yellow