SH800S

Citations:
Description:

The benchtop SH800S cell sorter permits sorting of a wide range of cell sizes and applications using the 70 μm, 100 μm, and 130 μm microfluidics sorting chips. This novel, chip-based design is fully integrated with comprehensive fluidics controls and advanced automation for set-up, acquisition, sort and analysis to make sorting less subjective, more precise and easier to use. System software is intuitive and supports sorting into tubes and 96- and 384-well plates. The software generates FCS 3.0 and FCS 3.1 files that also can be exported to third-party analysis tools.

Specifications
Sorter/ Analyzer

Cell Sorters

Supplier

Sony Biotechnology

Dimensions

55 x 55 x 72cm

RUO/Clinical

RUO

Colors (up to)

6

Lasers (up to)

4

Laser Options

VBYR

Customizable

No

Automation

Yes

Max Sample #

0

Sample Formats

Input: 0.5/1.5/5.0/15.0 mL tubes, Output: 1.5/5.0/15.0/50 mL tubes, multiwell plates

Sample Cooling

Yes

Events/Sec Max

100,000

Measuring/Sort Time

Using the 70-μm sorting chip at 50 kHz, an average threshold of 12,000 events per second can be achieved with >98% purity and >80% yield. A threshold rate up to 30,000 events per second can be achieved without affecting purity but with a decrease in yield based on Poisson’s statistics.

Detectors

8

Detector Type

PMT

Imaging

No

Microfluidic

Yes

Flat-top beam

No

Instrument Configuration
LASER
405
FILTERS
450/50

525/50

600/60

665/30

720/60

780/60
LASER
488
FILTERS
525/50

600/60

665/30

720/60

780/60
LASER
561
FILTERS
600/60

665/30

720/60

780/60
LASER
638
FILTERS
665/30

720/60

780/60