FX500

Citations:
Description:

The FX500 is a microfluidics chip based cell sorter. Its fully replaceable fluidics system lets researchers easily replace the entire fluidics path to control sample-to-sample carryover. The FX500 features comprehensive fluidics controls, advanced automation and easy to use software.

Patented CoreFinder™ technology automates instrument setup and streamlines workflow. The optical design offers up to three collinear excitation lasers - 488 nm, 561 nm, and 638 nm and six fluorescence detectors. The six free-form PMTs enable detection of fluorescence signals from any laser-based on filter setting.

The FX500 is for non-clinical research use only and is a Class 1 laser product.

Specifications
Sorter/ Analyzer

Cell Sorters

Supplier

Sony Biotechnology

Dimensions

55 x 55 x 72cm

RUO/Clinical

RUO

Colors (up to)

6

Lasers (up to)

3

Laser Options

BYR

Customizable

No

Automation

Optional

Max Sample #

0

Sample Formats

Input: 0.5/1.5/5.0/15.0 mL tubes, Output: 1.5/5.0/15.0/50 mL tubes, multiwell plates

Sample Cooling

Yes

Events/Sec Max

100,000

Measuring/Sort Time

Using the 70-μm sorting chip at 50 kHz, an average threshold of 12,000 events per second can be achieved with >98% purity and >80% yield. The threshold rate up to 30,000 events per second can be achieved without affecting purity but with a decrease in yield based on Poisson’s statistics.

Detectors

8

Detector Type

PMT

Imaging

No

Microfluidic

Yes

Flat-top beam

No

Colinear

Yes

Instrument Configuration
LASER
488
FILTERS
525/50

585/30

617/30

665/30

720/60

785/60