OMIP-048
Quantification of calcium sensors and channels expression in lymphocyte subsets by mass cytometry
Cytometry PART A, Volume 93, Issue 7, 681-684 (2018)
Agnieszka Jaracz‐Ros, Patrice Hémon, Roman Krzysiek, Françoise Bachelerie, Géraldine Schlecht‐Louf, Hélène Gary‐Gouy
Description: The present mass cytometry panel was designed to simultaneously assess the ex vivo expression of calcium (Ca2+)‐sensors and Ca2+‐channels, the key components of the T cells “Ca2+‐toolkit,” in murine conventional (Tconv) and Foxp3+ regulatory (Treg) T‐cell subsets, including both naive and activated populations, after a validation step of the selected antibodies by flow cytometry.
Species: Mouse
Tissue: Lymph node, Spleen
Cell Subset: T cell, T memory, Treg
Keyword: Calcium channel, Calcium sensor, Cytof
Machine: BD LSR Fortessa & Fluidigm CyTOF 1
Panel details
| Marker | Species | Clone | Fluorophore | Purpose | Co-expressions groups | Antigen Density |
|---|---|---|---|---|---|---|
| CD3 epsilon | Mouse | 145-2C11 | Purified | Lineage | ||
| CD19 | Mouse | 6D5 | Purified | Lineage | ||
| DNA | All Species | Purified | Nucleated cells | |||
| CD45R | Mouse | RA3-6B2 | Purified | Lineage | ||
| FOXP3 | Mouse | FJK-16s | Purified | Lineage | ||
| CD25 | Mouse | PC61 | Purified | Activation | ||
| ORAI2 | Mouse | Purified | Ca2+-channel | |||
| STIM2 | Mouse | Purified | Ca2+-sensor | |||
| DNA | All Species | Purified | Viability | |||
| CD4 | Mouse | RM4-5 | Purified | Lineage | ||
| CD44 | Mouse | IM7 | Purified | Activation | ||
| CD5 | Mouse | 53-7.3 | Purified | Lineage | ||
| CD62L | Mouse | Mel-14 | Purified | Activation | ||
| CD8a | Mouse | 53-6.7 | Purified | Lineage | ||
| VDAC1 | Mouse | 20B12AF2 | Purified | Ca2+-sensor/channel | ||
| STIM1 | Mouse | CDN3H4 | Purified | Ca2+-channel | ||
| ORAI1 | Mouse | 3F6H5 | Purified | Ca2+-channel | ||
| ORAI3 | Mouse | Purified | Ca2+-channel | |||
| Ki-67 | Mouse | B56 | Purified | Proliferation | ||
| CD45 | Mouse | 30-F11 | Purified | Lineage |
