Description: A 42‐parameter panel (34 antibody targets, cisplatin for live/dead, iridium for cell identification, and 6 barcodes) was developed for the characterization of cell types and signaling pathways present in tumor tissues or cell lines originating from primary, metastatic and recurrent head and neck squamous cell carcinoma (HNSCC), the most prevalent malignant tumor of the head and neck region and the 6th most common form of non‐skin cancer worldwide. Importantly, due to overlap in targets and markers across diverse tumor entities [e.g., HER2 amplification is present in both breast and gastric tumors], the described panel may be appropriate for use in preclinical models of additional distinct solid as well as hematological malignancies. This panel has been successfully tested on fresh, formalin‐fixed, and frozen cells originating from cell lines derived from primary and metastatic/recurrent tumors and from patients' tumor tissue.

Species: Human

Tissue: Cell line, Tumor

Cell Subset: Endothelial, Epithelial, B cell, T cell, T memory, Dc, Nk, Macrophage, Monocyte

Keyword: Activation, Cryopreserved tissue, Cytof, Fixed cells, Head and neck squamous cell carcinoma (hnscc), Immune checkpoint, Metastasis, Proliferation

Machine: Fluidigm CyTOF 2.1 Helios