OMIPs Directory

OMIP-081

A new 21-monoclonal antibody 10-color panel for diagnostic polychromatic immunophenotyping

Cytometry PART A, Volume 101, Issue 2, 117-121 (2022)
Erik H. L. P. G. Huys,Willemijn Hobo,Frank W. M. B. Preijers

Description: The 10-color panel consisting of 21 monoclonal antibodies (mAbs) is developed as a one-tube panel to detect leukemia and lymphoma cells in all hematopoietic cell lineages. In particular, this tube is mentioned for a fast screening to identify aberrant cells in samples suspected for malignant cell localization and to enable comprehensive immunophenotyping of samples with low cell counts. The panel contains mAbs for selection of the populations and mAbs against target antigens on the various hematopoietic maturation stages. Due to the limited number of PMTs in most used flow cytometers for clinical purposes, stacking of conjugates in one color is needed to include all relevant markers for simultaneous analysis of the aberrant cells. The 21-mAb panel is tested on peripheral blood (PB), and bone marrow (BM) samples and enables an efficient and correct identification of hematological malignancies. This panel improves the diagnostic potential.

Species: Human

Tissue: Bone marrow, Peripheral blood

Cell Subset: Plasma cell/ asc, B cell, Double negative b, T cell, Myeloid cells, Monocyte, B progenitor, Myeloid progenitor

Keyword: Leukemia, Lymphoma

Machine: Beckman Coulter Navios

Panel details

Marker Species Clone Fluorophore Purpose Co-expressions groups Antigen Density
CD3 Human UCHT1 APC-Alexa 750 Mature T cells T cell High
CD45 Human J33 Krome Orange WBC subpopulations Monocyte, T cell, NK High
CD8a Human B9.11 Pacific Blue Cytotoxic T cells, NK-cell subset T cell High
CD19 Human J3.119 ECD B-cell lineage B cell Low
Ig Lambda Light Chain Human Rabbit PE B-cell subset, B-cell lineage clonality B cell Med
CD7 Human 8H8.1 FITC T-cell lineage, T-cell activation, NK-cell subsets T cell, NK Med
CD138 Human B-B4 FITC Plasma cells B cell Med
CD36 Human FA6.152 APC-Alexa 700 Monocyte lineage, erythroid lineage, megakaryocytes Monocyte Med
CD10 Human ALB1 APC-Alexa 700 B-cell precursors, mature neutrophils B cell High
CD117 Human 104D2D1 APC Myeloid precursors Progenitor Low
CD34 Human 581 PE-Cy7 Progenitor cells Progenitor Med
CD33 Human D3HL60.251 PE-Cy5.5 Monocyte lineage, myeloid lineage Monocyte Med
CD20 Human B9E9 Pacific Blue Mature B cells B cell Med
CD56 Human NCAM16.2 PE NK cells, plasma cell subset NNK Low
CD5 Human BL1a PE-Cy5.5 T-cell lineage, B-cell subset B cell, T cell High
CD14 RMO52 ECD Mature monocytes
CD16 Human B73.1 PE T-cell subsets, NK cells, monocyte differentiation, eosinophil exclusion, myeloid lineage NK, Monocyte Med
CD15 Human 80H5 Pacific Blue Myeloid lineage High
CD4 Human SFCI12T4D11 PE-Cy7 Helper T cells, Monocyte lineage T cell High
CD2 Human 39C1.5 APC-Alexa 700 T-cell lineage, NK-cell subset T cell, NK Med
IG Kappa Light Chain Human Rabbit APC B-cell subset, B-cell lineage clonality B cell High