OMIP-011
Characterization of circulating endothelial cells (CECs) in peripheral blood
Cytometry PART A, Volume 81A, Issue 7, 549-551 (2012)
Raskit Lachmann, Paola Lanuti, Sebastiano Miscia
Description: This panel was optimized for the evaluation of circulating endothelial cells (CECs) in peripheral blood. The combination of three different endothelial cell markers enables a reasonable analysis of CECs. The panel, so far tested on fresh human peripheral and cord blood, can be used to enumerate CECs in a dual platform method.
Species: Human
Tissue: Cord blood, Peripheral blood
Cell Subset: Endothelial
Machine: BD FACSCanto 2
Panel details
| Marker | Species | Clone | Fluorophore | Purpose | Co-expressions groups | Antigen Density |
|---|---|---|---|---|---|---|
| Syto 16 | Human | Syto 16 | DNA counterstain | Endothelial, Progenitor | Unspecified | |
| CD45 | Human | HI30 | Alexa Fluor 700 | lymphocytes | High | |
| CD146 | Human | P1H12 | PE | endothelial cell marker | Endothelial | Low |
| CD117 | Human | 104D2 | PE-Cy7 | progenitor marker | Progenitor | Low |
| CD106 | Human | 51-10C9 | PE-Cy5 | activation | Endothelial | Low |
| Fixable viabilty | Human | Live/Dead Fix Near IR (775) | Viability | |||
| CD31 | Human | M89D3 | Alexa Fluor 647 | endothelial cell marker | Endothelial | High |
| CD34 | Human | 581 | ECD | endothelial cell marker | Progenitor, Endothelial | Med |
